Lancaster’s excellent run of 10 wins – seven in Division One, then three in the Super 5s – ended at Guildford when they were beaten 14-9 by Cheltenham on Sunday.
The weekend started off with City picking up a 15-11 win over City of Manchester.
Tom Curwen continued his outstanding form this season, scoring four times in the first quarter and adding another two more later in the game.
Glen Robinson bagged himself three goals as did fellow London 2012 Olympian Alex Parsonage.
Graham Harrington and Roger Kennedy put a goal away each and David Teasdale scored his first goal for Lancaster.
Sunday’s clash with Cheltenham was always going to be tough and was reflected in a scoreless opening quarter.
It was Cheltenham who scored the first five goals with Lancaster hitting back with three quick goals from Curwen, Robinson and Kennedy.
The third quarter saw four goals scored apiece from either team including a penalty awarded to both teams, Lancaster’s won by Lewis Peach and converted by Curwen.
Curwen knocked out another goal, along with Parsonage and Harrington.
Cheltenham went into the last quarter two goals up, in control of the game and Lancaster couldn’t find a way back.
Robinson and Parsonage scored another goal each but it was in vain as Lancaster’s winning run came to an end.
Coach Zak Sly said: “We have played outstandingly over the season losing one out of 11 games and we have a home advantage at our next weekend playing at Salt Ayre.
“The young team has bonded well and have it all to pay for in their next four games.”
The Super 5s continue at Salt Ayre Sports Centre, Lancaster, on December 6 and 7 with the hosts facing Bristol Central at 4.55pm on Saturday and Scottish side Porotbello at 11.20am on Sunday.
Entry is free with Lancaster hoping for a bumper home crowd.