Police are investigating a report that graffiti was sprayed inside the Carleton and items including a fridge, a chandelier and speakers were taken.
It is also alleged that CCTV cables were cut, a storeroom was broken into and a disabled toilet door handle and lock was removed.
A sign was also taken but this has now been recovered by police.
The Carleton has been closed since 2014 but reopened for one night only on January 30 2016 for a dance music night attended by 1,300 people.
A police spokesman said it is alleged the vandalism happened some time between January 30 and March 2.
No arrests have been made but an investigation is ongoing.
The iconic club is part of the Alhambra building on Marine Road West owned by developer Ian Bond.
Mr Bond’s long-term plan is to convert the former nightclub into flats.
He said the clean-up would cost £4,000 and he estimated the total damage and loss to be around £10,000.
Mr Bond also said once repairs were made, he would hire out the club for more events.
The next scheduled event at the Carleton is a ‘white-collar’ boxing show on June 4. An 80s night is also planned for sometime in July.